中职学校德育课课程改革初探

随着新一轮的中职学校德育课课程改革的深入开展和中职学生主体意识的增强,传统的德育课教学有许多方面需要进一步改进和完善.在中职学校德育课教学中实施高效良性的师生互动,能提高德育课的教学实效和学生的思想道德素质,为学生职业生涯发展奠定基础.     

积和式的几种常用计算方法

积和式是一种特殊的矩阵函数,它在组合设计、组合矩阵论和概率论等领域都有很广泛的应用,特别是在组合矩阵论中描述组合问题时更有着不可替代的优势.文章通过将积和式与行列式作类比,介绍了积和式的基本性质并研究了积和式的几种常用方法.     

固相萃取-亲水作用色谱法测定香菇中硫杂脯氨酸

通过对色谱条件和样品前处理方法进行优化,建立固相萃取—亲水作用色谱法测定香菇(Lentinula edodes)中硫杂脯氨酸的检测方法。优化后的色谱条件为：Inertsil HILIC 亲水作用色谱柱(4.6×250 mm,5μm)对样品进行分离测定,流动相采用乙腈-0.1%磷酸水(85∶15,v/v)等度洗脱,流速0.8 mL/min,检测波长193 nm,柱温30℃。前处理方法为：香菇样品采用甲醇-水(70∶30,v/v)提取,MAX阴离子固相萃取小柱进行净化处理,甲醇-1.0%甲酸(90∶10,v/v)作为洗脱剂对目标物进行洗脱。检测结果表明,硫杂脯氨酸在2~100μg/mL范围内呈良好的线性关系,相关系数为0.9998,加标回收率为96.3%~101.7%,相对标准偏差在1.3%~4.8%之间,检出限为0.76μg/g,定量限为2.31μg/g。该方法回收率高、简单、快速、无需衍生化,适用于香菇子实体硫杂脯氨酸的定量检测分析。     

粮食烘干塔换热器智能折边设备研发

结合螺旋板式换热器的应用领域及其板材折边生产现状,分析了其生产弊端。针对板材折边工艺要求,研发了粮食烘干塔换热器智能折边设备,并剖析其工作机理。根据典型产品结构参数,设计智能折边装备总体装配图并计算主要零件参数,为智能折边装备制作提供理论依据,从而实现换热器板材折边智能加工,提高换热器卷板折边质量及精度。     

Plant growth regulator and its interactions with environment and genotype affect maize optimal plant density and yield

Increasing planting density is important to raise maize yield, however, high density often leads to an increase risk of lodging due to dense canopy and weak stem. Maize yield and optimal plant density are increased by applying plant growth regulator compound of ethephon and DA-6, however, we do not know if this compound would interact with location and genotype. In this study, a novel plant growth regulator, as the synthesis of N, N- diethyl − 2 − hexanoyl oxygen radicals − ethyl amine (2-ethyl chloride) phosphonic acid salt (DHEAP), combining the effects of ethephon and DA-6 in one chemical, was developed and tested at three locations, five plant densities (6.75, 8.25, 9.75, 11.25 and 12.75 plants m⁻²) and three cultivars in 2014–2015. This study aimed to quantify the interactions between environment, genotype and management (Appling DHEAP and plant density) on lodging-related optimal plant density and yield. DHEAP significantly increased grain yield by 10.7% due to the increases of kernel weight by 3.2% and kernel number per ear by 4.4%. On average across genotypes and environments, applying DHEAP increased optimum plant density by 6%. The optimal plant density interacted with cultivar, DHEAP and environment. Applying DHEAP reduced lodging percentage by lowering ear height. The yield-lodging relationship was affected by genotype and location. We concluded that maize yield could be enhanced by optimizing plant density, applying DHEAP and cultivar selection, but climatic and environmental differences of locations should be considered.     

Molecular mapping of soybean seed tocopherols in the cross ‘OAC Bayfield’ × ‘OAC Shire’

Soybean (Glycine max [L.] Merr.) is cultivated primarily for its protein and oil in the seed. In addition, soybean seeds contain nutraceutical compounds such as tocopherols (vitamin E), which are powerful antioxidants with health benefits. The objective of this study was to identify molecular markers linked to quantitative trait loci (QTL) that affect accumulation of soybean seed tocopherols. A recombinant inbred line (RIL) population derived from the cross ‘OAC Bayfield’ × ‘OAC Shire’ was grown in three locations over 2 years. A total of 151 SSR markers were polymorphic of which a one‐way analysis of variance identified 42 markers whereas composite interval mapping identified 26 markers linked to tocopherol QTL across 17 chromosomes. Individual QTL explained from 7% to 42% of the total phenotypic variation. Significant two‐locus epistatic interactions were identified for a total of 122 combinations in 2009 and 152 in 2010. The multiple‐locus models explained 18.4–72.2% of the total phenotypic variation. The reported QTL may be used in marker‐assisted selection (MAS) to develop high tocopherol soybean cultivars.     

猪瘟病毒衣壳蛋白与宿主丝/苏氨酸蛋白激酶N1相互作用的鉴定

为筛选并鉴定与猪瘟病毒(CSFV)衣壳(C)蛋白相互作用的宿主蛋白,本研究采用酵母双杂交技术以CSFV C蛋白为诱饵从猪外周血单个核细胞(PBMC)c DNA表达文库中筛选与之相互作用的宿主蛋白,共筛选到6种蛋白,分别为ATP5B、SON3、PKN1、PCBP1、RPS20和IQGAP1,根据Gene Ontology分析结果,这些蛋白分别参与细胞的增殖和代谢等过程。本研究选取丝/苏氨酸蛋白激酶N1(PKN1)进行进一步验证,经酵母共转化试验、免疫共沉淀试验和GST pull-down试验证实,宿主PKN1蛋白与CSFV C蛋白之间存在特异性结合。本研究首次证明PKN1与CSFV蛋白之间的相互作用关系,为进一步研究PKN1蛋白在CSFV感染过程中发挥的作用奠定了基础。     

氮硫互作对大蒜植株生长的影响

为探究氮硫互作对大蒜植株生长的影响,以‘新疆白皮蒜’为试材,研究无机基质栽培条件下不同氮硫互作水平对大蒜农艺性状、叶绿素含量以及根系活力的影响。试验结果表明,不同生长阶段氮硫互作对大蒜株高、茎粗、植株鲜重、干重、鳞茎鲜重均有不同程度的增促效果,但氮或硫单一元素对其影响不一;大蒜生长过程中叶绿素含量与根系活力呈先升高后降低趋势,氮硫互作对其均有显著影响,且氮和硫素单一元素也显著影响大蒜根系活性。综合来看,大蒜生长过程中氮硫互作效果显著,在氮素20 mmol/L、硫素4 mmol/L条件下收获的鳞茎重量最大。     

巴西橡胶树乳管细胞HblMYC3互作蛋白筛选与功能分析

MYC是b HLH转录因子家族的亚家族成员,在植物茉莉酸信号转导过程中发挥着重要的调节作用。Hbl MYC3是从巴西橡胶树的乳管细胞中分离鉴定到的MYC类转录因子,其基因表达受割胶和茉莉酸上调。采用酵母双杂交方法初步筛选Hbl MYC3蛋白的互作蛋白,旨在进一步了解Hbl MYC3的功能。结果表明:Hbl MYC1、Hbl MYC2、DNAJ蛋白、谷氧还蛋白2、含A20和AN1锌指结构域的胁迫相关蛋白5、28 ku热和酸稳定的磷蛋白以及25 ku泛素连接酶E2等7种蛋白不同程度地与Hbl MYC3蛋白互作。基于这些互作蛋白的功能,推测Hbl MYC1或Hbl MYC2通过与Hbl MYC3形成二聚体对小橡胶粒子膜蛋白基因表达进行调控,其他蛋白参与胁迫条件下维持二聚体的稳定性和胁迫反应后降解该二聚体。